alleged national bikie boss accused of assaulting his partner has been refused bail in a Sydney court. Police say Mark Buddle has taken over as the presidents of the Comancheros bikie gang. The 27-year-old sat in the dock at Sydney's Central Local Court today, wearing the same T-shirt and shorts as when he was arrested on the street in the city's east at Coogee yesterday afternoon. Police allege Buddle had just slapped his de facto partner, Melanie Tiwacha, at their home at nearby Clovelly. He allegedly threatened the 27-year-old with a knife, slapped her and tried to stab her with a pen before she fled to a local pub and called police. Buddle was charged last night with assault, affray and two counts of being armed with intent. In applying for bail today, Buddle's lawyer Stephen Zahr told the court Ms Tiwacha had since moved in with her father. But Magistrate Julie Huber refused the bail application, saying she still had concerns for Ms Tiwacha's safety. Buddle is due to face court again next month.